Michigan Diabetes Research Center: MICPC: Imaging: Gallery

Three Dimensional Morphometric Analysis/Imaris

Three dimensional Measurements of Intraepidermal Nerve Fiber:

Fluorescent z-series images were collected on the Leica SP5 confocal microscope (nuclei of keratinocytes are shown as cyan). Intraepidermal nerve fibers (magenta) and branches (cyan) were rendered in 3D and measured with Imaris software.

Alexandra Münch, Nicholas Rebhan & Eva L. Feldman, M.D. Ph.D. Department of Neurology.

Three dimensional Measurements of Mitochondria within Intraepidermal Nerve Fibers:

Fluorescent z-series images were collected on the Leica SP5 confocal microscope (left side of the image, nerves green, mitochondria red and nuclei of keratinocytes are shown as cyan). On the right side of the image, intraepidermal nerve fibers (yellow) and mitochondria (red) were rendered in 3D and measured with Imaris software.

Hussein S. Hamid, M.D., John, M. Hayes & Eva L. Feldman, M.D. Ph.D. Department of Neurology, Stephen I. Lentz, Laboratory Director of Imaging of the MICPC.

Morphometric Analysis/MetaMorph

Fluorescence Recovery After Photo Bleaching:

   

Time series images were taken on the Olympus FV500 confocal microscope. The fluorescent signals within the regions of interest of the bleached area (green), ajacent to the bleached area (yellow), whole cell (magenta) or outside the cell (red) were measured over time with MetaMorph software. Cells expressing green fluorescent markers were provided and imaged by Stephen A. Ernst, Ph.D., Department of Cell and Developmental Biology and Edward L. Stuenkel, Department of Molecular and Integrative Physiology.

Time series images were taken on the Leica SP5 confocal microscope. The fluorescent signals within the regions of interest of the bleached area (S1:1 red, S2:2 green, and S3:3 blue) and away from the bleached area (R:4 yellow, R:5 magenta, and R:6 cyan were measured over time with MetaMorph software. Madin-Darby canine kidney (MDCK) epithelial cells expressing green fluorescent markers were provided and imaged by Mark T. Bolinger, Ph.D., and David A. Antonetti, Ph.D., Department of Ophthalmology and Visual Sciences.

Mitochondria Trafficking:

Time series images were taken on the Nikon A1 confocal microscope. Mitochondria were labeled with a fluorescent signals in murine primary sensory neurons. Mitochondria trafficking in neurites was done with kymograph analysis using MetaMorph software. Primary senosry neuron cultures were provided and imaged by Amy Rumora, Eva L. Feldman, M.D. Ph.D., Department of Neurology and Stephen I. Lentz, Ph.D., Laboratory Director of Imaging, MICPC.

G-ratio Measurements in Mouse Seral Nerve

G-ratio (the ratio of the inner axonal diameter to the total outer diameter) is a highly reliable ratio for assessing axonal myelination. TEM images were analyzed with MetaMorph Software by John M. Hayes, B.S., Andrew Solway, Lucy M. Hinder and Eva L. Feldman, M.D., Ph.D., Department of Neurology.

Adipocyte Measurements:

Original images were collected on a Nikon microscope. Image contrast was enhanced with PhotoShop. Adipocyte area and number were measured with MetaMorph software and data was plotted with Prism software by Tyler C. Prestwich, Sebastian D. Parlee, Ph.D. and Ormond A. MacDougald, Ph.D. Department of Molecular and Integrative Physiology.