Michigan Diabetes Research Center: MICPC: In Situ Hybridization

Director: Carol F. Elias, PhD
Associate Professor, Molecular and Integrative Physiology
Med Sci II Room 7732B
Phone: 734-647-2801
Email: cfelias@umich.edu

The in situ hybridization (ISH) core provides several methods of localization and quantification of RNA via the use of riboprobes. Different detection methods are available according to riboprobes labeling:

Hybridization Image
  1. Radioisotopes: autoradiographic film and emulsion
  2. Digoxigenin: alkaline-phosphatase and immunoperoxidase
  3. Biotin: immunoperoxidase and immunofluorescence
  4. Fluorescence: RNAprobe using RNAscope technology*

The core provides the following services:

  1. Consultation
  2. cDNA production
  3. Probe labeling by in vitro transcription
  4. Tissue processing
  5. Complete ISH procedure (hybridization and signal detection)
  6. Dual label ISH / immunohistochemistry
  7. Data analysis
  8. Training

Cost of services:

Single ISHH Digoxigenin
$216 per ISH assay (Alkaline-phosphatase or Immunoperoxidase)
Single ISHH Biotin
$216 per ISH assay (Immunoperoxidase or Immunofluorescence)
Single ISHH 35S
$407 per ISH assay
Single ISHH 33P
$599 per ISH assay
Single ISHH Sample Sectioning/Mounting
$265 per ISH assay
Antibodies for Double Label
$30 per ISHH assay

Service Agreement/Application

Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”