Michigan Diabetes Research Center: MICPC: In Situ Hybridization

Director: Carol F. Elias, Ph.D.
Associate Professor, Molecular and Integrative Physiology
Med Sci II Room 7732B
Phone: 734-647-2801
cfelias@umich.edu

The in situ hybridization (ISH) core provides several methods of localization and quantification of RNA via the use of riboprobes. Different detection methods are available according to riboprobes labeling:

Hybridization Image
  1. Radioisotopes: autoradiographic film and emulsion
  2. Digoxigenin: alkaline-phosphatase and immunoperoxidase
  3. Biotin: immunoperoxidase and immunofluorescence
  4. Fluorescence: RNAprobe using RNAscope technology*

The core provides the following services:

  1. Consultation
  2. cDNA production
  3. Probe labeling by in vitro transcription
  4. Tissue processing
  5. Complete ISH procedure (hybridization and signal detection)
  6. Dual label ISH / immunohistochemistry
  7. Data analysis
  8. Training

Cost of services:

Single ISHH Dig
$1,245.47 per ISH assay
Single ISHH 35S
$1,328.17 per ISH assay
Single ISHH 33P
$1,420.42 per ISH assay
Single ISHH Sample Sectioning/Mounting
$276.00 per ISH assay

* Contact: David Antonetti, Ph.D.
Brehm Tower, dantonet@med.umich.edu, 724-232-8230